Abstract
This chapter focuses on visualization of cell cycle progression in plant tissues using the dual-color marker system “Cytrap.” The Cytrap line carries a part of Arabidopsis CDT1a fused to the red fluorescent protein (RFP) gene, which monitors the cell cycle phases from S to late G2 or early mitosis, and the G2/M-specific cyclin B1 marker fused to the green fluorescent protein (GFP) gene. We introduce growth conditions of Arabidopsis roots, the setup for microscopic observation, and analysis of obtained images.
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Aki, S.S., Umeda, M. (2016). Cytrap Marker Systems for In Vivo Visualization of Cell Cycle Progression in Arabidopsis . In: Caillaud, MC. (eds) Plant Cell Division. Methods in Molecular Biology, vol 1370. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3142-2_4
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DOI: https://doi.org/10.1007/978-1-4939-3142-2_4
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3141-5
Online ISBN: 978-1-4939-3142-2
eBook Packages: Springer Protocols