Skip to main content

Nanotechnology in the Fabrication of Protein Microarrays

  • Protocol

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1368))

Abstract

Protein biochips are the heart of many medical and bioanalytical applications. Increasing interest of protein biochip fabrication has been focused on surface activation and subsequent functionalization strategies for the immobilization of these molecules.

This is a preview of subscription content, log in via an institution.

Buying options

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Vaisocherová H, Zhang Z, Yang W et al (2009) Functionalizable surface platform with reduced nonspecific protein adsorption from full blood plasma-Material selection and protein immobilization optimization. Biosens Bioelectron 24:1924–1930

    Article  PubMed  Google Scholar 

  2. Valiokas R (2012) Nanobiochips. Cell Mol Life Sci 69:347–356

    Article  CAS  PubMed  Google Scholar 

  3. Romanov V, Davidoff SN, Miles AR et al (2014) A critical comparison of protein microarray fabrication technologies. Analyst 139:1303–1326

    Article  CAS  PubMed  Google Scholar 

  4. Casado-Vela J, González-González M, Matarraz S et al (2013) Protein arrays: recent achievements and their application to study the human proteome. Curr Proteomics 10:83–97

    Article  CAS  Google Scholar 

  5. Tsarfati-Barad I, Sauer U, Preininger C et al (2011) Miniaturized protein arrays: model and experiment. Biosens Bioelectron 26:3774–3781

    Article  CAS  PubMed  Google Scholar 

  6. Gonzalez-Gonzalez M, Bartolome R, Jara-Acevedo R et al (2014) Evaluation of homo- and hetero-functionally activated glass surfaces for optimized antibody arrays. Anal Biochem 450:37–45

    Article  CAS  PubMed  Google Scholar 

  7. Biebricher A, Paul A, Tinnefeld P et al (2004) Controlled three-dimensional immobilization of biomolecules on chemically patterned surfaces. J Biotechnol 112:97–107

    Article  CAS  PubMed  Google Scholar 

  8. Jonkheijm P, Weinrich D, Schröder H et al (2008) Chemical strategies for generating protein biochips. Angew Chem Int Ed 47:9618–9647

    Article  CAS  Google Scholar 

  9. Tien J, Xia Y, Whitesides GM (1998) Microcontact printing of SAMs. Thin Films 24:227–250

    CAS  Google Scholar 

  10. Cras JJ, Rowe-Taitt CA, Nivens DA et al (1999) Comparison of chemical cleaning methods of glass in preparation for silanization. Biosens Bioelectron 14:683–688

    Article  CAS  Google Scholar 

  11. Krasnoslobodtsev AV, Smirnov SN (2002) Effect of water on silanization of silica by trimethoxysilanes. Langmuir 18:3181–3184

    Article  CAS  Google Scholar 

  12. Kyprianou D, Guerreiro AR, Chianella I et al (2009) New reactive polymer for protein immobilisation on sensor surfaces. Biosens Bioelectron 24:1365–1371

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

We gratefully acknowledge financial support from the Carlos III Health Institute of Spain (ISCIII, FIS PI11/02114, FIS14/01538, Fondos FEDER-EU) and Junta Castilla-León SA198A12-2. The Proteomics Unit belongs to ProteoRed, PRB2-ISCIII, as supported by grant PT13/0001. P. Díez is supported by a JCYL-EDU/346/2013 PhD scholarship. J. Casado-Vela is a JAE-DOC (CSIC) holder supported by Ministerio de Economía y Competitividad, Spain, co-funded by the European Social Fund (Fondos FEDER).

Author information

Authors and Affiliations

Authors

Corresponding authors

Correspondence to Manuel Fuentes or Juan Casado-Vela .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2016 Springer Science+Business Media New York

About this protocol

Cite this protocol

Fuentes, M., Díez, P., Casado-Vela, J. (2016). Nanotechnology in the Fabrication of Protein Microarrays. In: Li, P., Sedighi, A., Wang, L. (eds) Microarray Technology. Methods in Molecular Biology, vol 1368. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3136-1_14

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-3136-1_14

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3135-4

  • Online ISBN: 978-1-4939-3136-1

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics