Abstract
Technological advances that allow deeper penetration in live tissues, such as the development of confocal and the generation of ever-new fluorophores that facilitate bright labeling of cells and tissue components have made imaging of vertebrate model organisms efficient and highly informative. Recently, high luminescence, single-excitation narrow emission, low photo bleaching properties, and low toxicity of high-quality water-soluble QDs have attracted attention for in vivo labeling/imaging of cells. Herein we describe a synthetic approach to biotinylated glycopolymer functionalized quantum dots, with special emphasis on the development of high-quality water-soluble and bioactive QDs with low toxicity for fluorescent probes in biomedical applications.
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References
Bradburne CE, Delehanty JB, Gemmill KB, Mei BC, Mattoussi H, Susumu K et al (2013) Cytotoxicity of quantum dots used for in vitro cellular labeling: role of QD surface ligand, delivery modality, cell type, and direct comparison to organic fluorophores. Bioconjugate Chem 24:1570–1583
Algar WR, Susumu K, Delehanty JB, Medintz IL (2011) Semiconductor quantum dots in bioanalysis: crossing the valley of death. Anal Chem 83:8826–8837
Delehanty JB, Mattoussi H, Medintz IL (2009) Delivering quantum dots into cells: strategies, progress and remaining issues. Anal Bioanal Chem 393:1091–1105
Delehanty JB, Susumu K, Manthe RL, Algar WR, Medintz IL (2012) Active cellular sensing with quantum dots: transitioning from research tool to reality; a review. Anal Chim Acta 750:63–81
Algar WR, Prasuhn DE, Stewart MH, Jennings TL, Blanco-Canosa JB, Dawson PE, Medintz IL (2011) The controlled display of biomolecules on nanoparticles: a challenge suited to bioorthogonal chemistry. Bioconjugate Chem 22:825–858
Jiang X, Ahmed M, Deng Z, Narain R (2009) Biotinylated glyco-functionalized quantum dots: synthesis, characterization, and cytotoxicity studies. Bioconjugate Chem 20:994–1001
Deng Z, Ahmed M, Narain R (2009) Novel well-defined glycopolymers synthesized via the reversible addition fragmentation chain transfer process in aqueous media. J Pol Sci A Pol Chem 47:614–627
Deng Z, BouchéKif H, Babooram K, Housni A, Choytun N, Narain R (2008) Facile Synthesis of controlled-structure primary amine-based methacrylamide polymers via the reversible addition-fragmentation chain transfer process. J Pol Sci A Pol Chem 46:4984–4996
Lai JT, Filla D, Shea R (2002) Functional polymers from novel carboxyl-terminated trithiocarbonates as highly efficient RAFT agents. Macromolecules 35:6754–6756
Narain R, Armes SP (2002) Synthesis of low polydispersity, controlled-structure sugar methacrylate polymers under mild conditions without protecting group chemistry. Chem Commun 23:2776–2777
Green NM (1965) A spectrophotometric assay for avidin and biotin based on binding of dyes by avidin. Biochem J 94:23c–24c
Green NM (1970) Spectrophotometric determination of avidin and biotin. Methods Enzymol 18:418–424
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Adokoh, C.K., Darkwa, J., Narain, R. (2016). Synthetic Approach to Biotinylated Glyco-Functionalized Quantum Dots: A New Fluorescent Probes for Biomedical Applications. In: Sun, XL. (eds) Macro-Glycoligands. Methods in Molecular Biology, vol 1367. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3130-9_9
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DOI: https://doi.org/10.1007/978-1-4939-3130-9_9
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3129-3
Online ISBN: 978-1-4939-3130-9
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