Abstract
Proteoglycans are proteins with pendant glycosaminoglycan polysaccharide side chains. The method described here enables the preparation of graft copolymers with glycosaminoglycan side chains, which mimic the structure and composition of proteoglycans. By controlling the stoichiometry, graft copolymers can be obtained with a wide range of glycosaminoglycan side-chain densities. The method presented here uses a three-step reaction mechanism to first functionalize a hyaluronic acid backbone, followed by reductive amination to couple the glycosaminoglycan side chain to the backbone, by the reducing end. Proteoglycan mimics like the ones proposed here could be used to study the structure–property relationships of proteoglycans and to introduce the biochemical and biomechanical properties of proteoglycans into biomaterials and therapeutic formulations.
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Acknowledgements
Sean M. Kelly contributed to the successful demonstration of this technique. We thank Prof. Patrick A. Johnson (University of Wyoming) for access to dynamic light scattering and electrophoretic mobility instrumentation, Prof. Travis S. Bailey (Colorado State University) for helpful discussions, and Prof. Melissa M. Reynolds and Alec Lutzke for assistance with ATR-FTIR. Funding for the original work done to develop this protocol was provided by the National Science Foundation (DMR 0847641).
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Kipper, M.J., Place, L.W. (2016). Preparation of Proteoglycan Mimetic Graft Copolymers. In: Sun, XL. (eds) Macro-Glycoligands. Methods in Molecular Biology, vol 1367. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3130-9_7
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DOI: https://doi.org/10.1007/978-1-4939-3130-9_7
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