Abstract
Typical synthetic methods for glycopolymers are laborious and require multistep processes, including protection and deprotection steps. Here we describe a facile protecting-group-free synthetic approach to glycopolymers bearing oligosaccharides from free saccharides by direct azidation and click chemistry methods, followed by reversible addition-fragmentation chain transfer polymerization. This method can be applied not only to mono- and disaccharides, but also to large biologically relevant oligosaccharides having sialic acids. Due to the glycocluster effect, the glycopolymers strongly bind with the corresponding lectin and influenza A virus, as analyzed by the quartz crystal microbalance method and hemagglutination inhibition assay.
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Acknowledgment
This work was financially supported by JSPS KAKENHI Grant No. 25810075.
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Tanaka, T., Takahashi, T., Suzuki, T. (2016). Protecting-Group-Free Synthesis of Glycopolymers and Their Binding Assay with Lectin and Influenza Virus. In: Sun, XL. (eds) Macro-Glycoligands. Methods in Molecular Biology, vol 1367. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3130-9_4
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DOI: https://doi.org/10.1007/978-1-4939-3130-9_4
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3129-3
Online ISBN: 978-1-4939-3130-9
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