Abstract
We describe a protocol for the preparation of glycopolymers derived from the ring-opening polymerization of a norbornene carboxylic acid derivative. Polymerization is followed by attachment of a linker and subsequent glycoconjugation via a triazole-forming azide-alkyne click reaction. The use of a protected amine-terminating agent allows for the attachment of a probe molecule such as a fluorescein dye. The syntheses of a neutral galactopolymer as well a polyanionic poly-3-O-sulfo-galactopolymer are described.
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Acknowledgement
This work was supported by the National Science Foundation (CHE-0910947).
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Okoth, R., Basu, A. (2016). Glycopolymers Prepared by Ring-Opening Metathesis Polymerization Followed by Glycoconjugation Using a Triazole-Forming “Click” Reaction. In: Sun, XL. (eds) Macro-Glycoligands. Methods in Molecular Biology, vol 1367. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3130-9_3
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DOI: https://doi.org/10.1007/978-1-4939-3130-9_3
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3129-3
Online ISBN: 978-1-4939-3130-9
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