Abstract
Regulation of Activation Induced Deaminase (AID) by the hormone estrogen has important implications for understanding adaptive immune responses as well as the involvement of AID in autoimmune diseases and tumorigenesis. This chapter describes the general laboratory techniques for analyzing AID expression and activity induced by estrogen, focusing on the isolation and preparation of cells for hormone treatment and the subsequent analysis of AID responsiveness to estrogen at the RNA level and for determining the regulation of AID activity via estrogen by analyzing Ig switch circle transcripts and mutations in switch region loci.
The original version of this chapter was revised. The erratum to this chapter is available at: DOI 10.1007/978-1-4939-3127-9_44
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Acknowledgement
S.P. was supported by FEBS long-term fellowship.
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Pauklin, S. (2016). Regulation of Activation Induced Deaminase (AID) by Estrogen. In: Eyster, K.M. (eds) Estrogen Receptors. Methods in Molecular Biology, vol 1366. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3127-9_43
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DOI: https://doi.org/10.1007/978-1-4939-3127-9_43
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3126-2
Online ISBN: 978-1-4939-3127-9
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