Detection of the Phosphorylation of the Estrogen Receptor α as an Outcome of GPR30 Activation
Phosphorylation of the serine residues in estrogen receptor (ER) α is important in transcriptional activation. Hence, methods to detect such posttranslational modification events are valuable. We describe, in detail, the analysis of the phosphorylated ERα by electrophoretic separation of proteins and subsequent immuno-blotting techniques. In particular, phosphorylation of the ERα is one possible outcome of activation of the putative membrane estrogen receptor (mER), GPR30. Hence, phosphorylation represents a cross talk event between GPR30 and ERα and may be important in estrogen-regulated physiology.
Key wordsPhosphorylation Serine Nuclear receptor Antibody Tissue Lysate Cell line GPR30 ERα
This work was supported by funding supplied by the NSF CAREER IOS-1053716 to N.V. and a Louisiana Board of Regents Fellowship to K.P.
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