Comparison of the Effects of the Selective Estrogen Receptor Modulators Ospemifene, Raloxifene, and Tamoxifen on Breast Tissue in Ex Vivo Culture

  • Natalija Eigeliene
  • Risto Erkkola
  • Pirkko Härkönen
Part of the Methods in Molecular Biology book series (MIMB, volume 1366)


Explant tissue culture provides a model for studying the direct effects of steroid hormones, their analogs, and novel hormonally active compounds on normal freshly isolated human breast tissues (HBTs). For this purpose, pre- and postmenopausal HBTs can be maintained in this culture system. The results demonstrate that the morphological integrity of HBT explants can be maintained in tissue culture up to 2 weeks and expression of differentiation markers, steroid hormone receptors, proliferation and apoptosis ratios can be evaluated as a response to hormonal stimulation. This chapter describes an ex vivo culture model that we have applied to study the effects of various hormonally active substances, including 17β-estradiol and selective estrogen receptor modulators (SERMs), on normal human breast tissues.

Key words

Human breast tissue Explant tissue culture Estradiol Estrogenreceptoralpha SERMs 



This work was supported by the Turku University Hospital, the Academy of Finland, and the Sigfrid Jusélius Foundation.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Natalija Eigeliene
    • 1
  • Risto Erkkola
    • 2
  • Pirkko Härkönen
    • 1
  1. 1.Department of Cell Biology and Anatomy, Institute of BiomedicineUniversity of TurkuTurkuFinland
  2. 2.Department of Obstetrics and GynecologyTurku University Central HospitalTurkuFinland

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