Skip to main content
SpringerLink
Log in
Book cover

Estrogen Receptors pp 315–325Cite as

Use of Reporter Genes to Analyze Estrogen Response: The Transgenic Zebrafish Model

  • Protocol

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1366))

Abstract

In vivo models to detect estrogenic compounds are very valuable for screening for endocrine disruptors. Here we describe the use of transgenic estrogen reporter zebrafish as an in vivo model for identification of estrogenic properties of compounds. Live imaging of these transgenic fish provides knowledge of estrogen receptor specificity of different ligands as well as dynamics of estrogen signaling. Coupled to image analysis, the model can provide quantitative dose-response information on estrogenic activity of chemical compounds.

This is a preview of subscription content, log in via an institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   119.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Bridgham JT, Eick GN, Larroux C et al (2010) Protein evolution by molecular tinkering: diversification of the nuclear receptor superfamily from a ligand-dependent ancestor. PLoS Biol 8:e1000497

    Article  Google Scholar 

  2. Eick GN, Thornton JW (2011) Evolution of steroid receptors from an estrogen-sensitive ancestral receptor. Mol Cell Endocrinol 334:31–38

    Article  CAS  Google Scholar 

  3. Bondesson M, Hao R, Lin CY et al (2014) Estrogen receptor signaling during vertebrate development. Biochim Biophys Acta 1849(2):142–151

    Article  Google Scholar 

  4. Huang R, Sakamuru S, Martin MT et al (2014) Profiling of the Tox21 10K compound library for agonists and antagonists of the estrogen receptor alpha signaling pathway. Sci Rep 4:5664

    Article  CAS  Google Scholar 

  5. Gerety SS, Breau MA, Sasai N et al (2013) An inducible transgene expression system for zebrafish and chick. Development 140:2235–2243

    Article  CAS  Google Scholar 

  6. Gorelick DA, Halpern ME (2011) Visualization of estrogen receptor transcriptional activation in zebrafish. Endocrinology 152:2690–2703

    Article  CAS  Google Scholar 

  7. Legler J, Zeinstra LM, Schuitemaker F et al (2002) Comparison of in vivo and in vitro reporter gene assays for short-term screening of estrogenic activity. Environ Sci Technol 36:4410–4415

    Article  CAS  Google Scholar 

  8. Tong SK, Mouriec K, Kuo MW et al (2009) A cyp19a1b-gfp (aromatase B) transgenic zebrafish line that expresses GFP in radial glial cells. Genesis 47:67–73

    Article  CAS  Google Scholar 

  9. Gorelick DA, Iwanowicz LR, Hung AL et al (2014) Transgenic zebrafish reveal tissue-specific differences in estrogen signaling in response to environmental water samples. Environ Health Perspect 122:356–362

    CAS  PubMed  PubMed Central  Google Scholar 

  10. Hao R, Bondesson M, Singh AV et al (2013) Identification of estrogen target genes during zebrafish embryonic development through transcriptomic analysis. PLoS One 8:e79020

    Article  CAS  Google Scholar 

  11. Avdesh A, Chen M, Martin-Iverson MT et al (2012) Regular care and maintenance of a zebrafish (Danio rerio) laboratory: an introduction. J Vis Exp e4196

    Google Scholar 

  12. Lawrence C, Best J, James A et al (2012) The effects of feeding frequency on growth and reproduction in zebrafish (Danio rerio). Aquaculture 368–369:103–108

    Article  Google Scholar 

  13. Mandrell D, Truong L, Jephson C et al (2012) Automated zebrafish chorion removal and single embryo placement: optimizing throughput of zebrafish developmental toxicity screens. J Lab Autom 17:66–74

    Article  Google Scholar 

  14. Matos-Cruz V, Blasic J, Nickle B et al (2011) Unexpected diversity and photoperiod dependence of the zebrafish melanopsin system. PLoS One 6:e25111

    Article  CAS  Google Scholar 

  15. Pinto C, Grimaldi M, Boulahtouf A et al (2014) Selectivity of natural, synthetic and environmental estrogens for zebrafish estrogen receptors. Toxicol Appl Pharmacol 280:60–69

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The data presented here were generated in projects funded by grants from the Environmental Protection Agency (R834289), NIH (F32HD061119) and startup funds from Dept. of Pharmacology & Toxicology at the University of Alabama at Birmingham.

Author information

Authors and Affiliations

  1. Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL, USA

    Daniel A. Gorelick

  2. Center for Nuclear Receptors and Cell Signaling, Department of Biology and Biochemistry, University of Houston, 4800 Calhoun Rd., Houston, TX, 77004, USA

    Caroline Lucia Pinto & Maria Bondesson

  3. Center for Molecular Toxicology and Carcinogenesis, Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA, USA

    Ruixin Hao

  4. DuPont Haskell Global Centers for Health and Environmental Sciences, Newark, DE, USA

    Ruixin Hao

Authors
  1. Daniel A. Gorelick
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Caroline Lucia Pinto
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ruixin Hao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Maria Bondesson
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Maria Bondesson .

Editor information

Editors and Affiliations

  1. University of South Dakota Sanford School of Medicine, Vermillion, South Dakota, USA

    Kathleen M. Eyster

Rights and permissions

Reprints and permissions

Copyright information

© 2016 Springer Science+Business Media New York

About this protocol

Cite this protocol

Gorelick, D.A., Pinto, C.L., Hao, R., Bondesson, M. (2016). Use of Reporter Genes to Analyze Estrogen Response: The Transgenic Zebrafish Model. In: Eyster, K.M. (eds) Estrogen Receptors. Methods in Molecular Biology, vol 1366. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3127-9_24

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-3127-9_24

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3126-2

  • Online ISBN: 978-1-4939-3127-9

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation