Abstract
This chapter describes protocols for using a handheld gene gun to deliver transformation vectors for overexpression of genes or gene replacement in the macronucleus of Tetrahymena thermophila. The protocols provide helpful information for preparing Tetrahymena for biolistic bombardment, preparation of vector-coated microcarriers, and basic gene gun operating procedures.
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Acknowledgements
The authors thank Roland E. Hosein for laboratory assistance. Protocols in this chapter are based on research performed at Brooklyn College-CUNY and supported by Grants MCB 0517083 and DBI 0619460 from National Science Foundation awarded to Dr. Ray H. Gavin.
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Gotesman, M., Williams, S.A. (2016). Using a Handheld Gene Gun for Genetic Transformation of Tetrahymena thermophila . In: Gavin, R. (eds) Cytoskeleton Methods and Protocols. Methods in Molecular Biology, vol 1365. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3124-8_22
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DOI: https://doi.org/10.1007/978-1-4939-3124-8_22
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3123-1
Online ISBN: 978-1-4939-3124-8
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