Abstract
Using Drosophila actin-binding protein Dunc-115 as model system, this chapter describes a MARCM (mosaic analysis with a repressible cell marker)-based method for analyzing cytoskeletal components for their functions in the nervous system. Following a concise description about the principle, a step-by-step protocol is provided for generating the needed stocks and for histological analysis. Additional details and explanations have been given in the accompanying notes. Together, this should form a practical and sufficient recipe for performing at the single-cell-level loss-of-function and gain-of-function analyses of proteins associated with the cytoskeleton.
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Acknowledgments
I would like to thank the Bloomington Stock Center for providing fly strains and Ray Gavin for reviewing the manuscript. This work was supported in part by a PSC-CUNY grant.
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He, Q., Roblodowski, C. (2016). Functional Analysis of Actin-Binding Proteins in the Central Nervous System of Drosophila . In: Gavin, R. (eds) Cytoskeleton Methods and Protocols. Methods in Molecular Biology, vol 1365. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3124-8_20
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DOI: https://doi.org/10.1007/978-1-4939-3124-8_20
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3123-1
Online ISBN: 978-1-4939-3124-8
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