Abstract
Variable angle total internal reflection fluorescence microscopy (VA-TIRFM) is an optical method to observe the molecular events occurring in an extremely thin region near the plasma membrane. Recently, the VA-TIRFM technique has been widely used to study fluorescently labeled target molecules in living animal and plant cells. Here, we describe the optical principle of the VA-TIRFM technique and provide a detailed experimental procedure for the study of living plant cells.
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Acknowledgments
Our works were supported by the Fundamental Research Funds for the Central Universities (JC2013-2), Program for New Century Excellent Talents in University (NCET-12-0785), Key Grant Project of Chinese Ministry of Education (No. 313008), and the National Natural Science Foundation of China (31271433).
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Wan, Y., Xue, Y., Li, R., Lin, J. (2016). Application of Variable Angle Total Internal Reflection Fluorescence Microscopy to Investigate Protein Dynamics in Intact Plant Cells. In: Botella, J., Botella, M. (eds) Plant Signal Transduction. Methods in Molecular Biology, vol 1363. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3115-6_10
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DOI: https://doi.org/10.1007/978-1-4939-3115-6_10
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3114-9
Online ISBN: 978-1-4939-3115-6
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