Abstract
Engineered PEG-cleavable catiomers based on poly-l-lysine have been developed as nonviral gene vectors, which have been found to be one of important methods to balance “PEG dilemma.” In this protocol, we aim at the standardization of the method and procedure of PEG-cleavable catiomers. Major steps including ring-opening polymerization (ROP) of ε-benzyloxycarbonyl-l-lysine N-carboxyanhydride (zLL-NCA) monomers to yield PEG-cleavable polylysine, examination on bio-stability and bio-efficacy of its gene complexes are described.
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Acknowledgement
This work was financially supported by research grants from the National Natural Science Foundation of China (NSFC 21104059 and 51173136), the Fundamental Research Funds for the Central Universities (2013KJ038), and “Chen Guang” project (12CG17) founded by Shanghai Municipal Education Commission and Shanghai Education Development Foundation.
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Tang, M., Dong, H., Cai, X., Zhu, H., Ren, T., Li, Y. (2016). Disulfide-Bridged Cleavable PEGylation of Poly-l-Lysine for SiRNA Delivery. In: Shum, K., Rossi, J. (eds) SiRNA Delivery Methods. Methods in Molecular Biology, vol 1364. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3112-5_5
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DOI: https://doi.org/10.1007/978-1-4939-3112-5_5
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3111-8
Online ISBN: 978-1-4939-3112-5
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