Abstract
Data analysis is essential to derive meaningful conclusions from proteomic data. This chapter describes ways of performing common data visualization and differential analysis tasks on gel-based proteomic datasets using a freely available statistical software package (R). A workflow followed is illustrated using a synthetic dataset as example.
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References
Dowsey AW, Morris JS, Gutstein HB etal (2010) Informatics and statistics for analyzing 2-D gel electrophoresis images. In: Hubbard JS, Jones AR (eds) Proteome bioinformatics. Humana Press, NewYork, pp239–255
Silva TS, Richard N, Dias JP etal (2014) Data visualization and feature selection methods in gel-based proteomics. Curr Protein Pept Sci 15:4–22
Hothorn T, Everitt B (2009) A handbook of statistical analyses using R, 2nd edn. Chapman & Hall/CRC, London
Crawley MJ (2012) The R book. Wiley, Chichester, England
Dowsey AW, English JA, Lisacek F etal (2010) Image analysis tools and emerging algorithms for expression proteomics. Proteomics 10:4226–4257
Rye M, Fargestad EM (2012) Preprocessing of electrophoretic images in 2-DE analysis. Chemometr Intell Lab Syst 117:70–79
Wheelock M, Buckpitt AR (2005) Software-induced variance in two-dimensional gel electrophoresis image analysis. Electrophoresis 26:4508–4520
Chich J-F, David O, Villers F etal (2007) Statistics for proteomics: experimental design and 2-DE differential analysis. J Chromatogr B 849:261–272
Smyth GK (2004) Linear models and empirical Bayes methods for assessing differential expression in microarray experiments. Stat Appl Genet Mol Biol 3:1–25
Storey JD, Tibshirani R (2003) Statistical significance for genomewide studies. Proc Natl Acad Sci U S A 100:9440–9445
Artigaud S, Gauthier O, Pichereau V (2013) Identifying differentially expressed proteins in two-dimensional electrophoresis experiments: Inputs from transcriptomics statistical tools. Bioinformatics 29:2729–2734
Cao K-AL, Boitard S, Besse P (2011) Sparse PLS discriminant analysis: biologically relevant feature selection and graphical displays for multiclass problems. BMC Bioinformatics 12:253
Guyon I, Elisseeff A (2003) An introduction to variable and feature selection. J Mach Learn Res 3:1157–1182
Acknowledgement
Nadège Richard was supported by a postdoctoral grant (SFRH/BDP/65578/2009) from the Portuguese Foundation for Science and Technology (FCT).
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Silva, T.S., Richard, N. (2016). Visualization and Differential Analysis of Protein Expression Data Using R. In: Jung, K. (eds) Statistical Analysis in Proteomics. Methods in Molecular Biology, vol 1362. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3106-4_6
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DOI: https://doi.org/10.1007/978-1-4939-3106-4_6
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3105-7
Online ISBN: 978-1-4939-3106-4
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