Abstract
NanoString nCounter is a recently developed platform that can make direct multiplexed measurement of gene expression using color-coded probe pairs (Geiss et al., Nat Biotechnol 26(3):317–325, 2008; Malkov et al., BMC Res Notes 2:80, 2009). We have found that this platform is uniquely suitable for quantification of pathogen gene expression during infection, where pathogen RNA comprises a tiny portion of total RNA isolated from the infected tissue. Here, we describe a protocol that we have successfully applied to a number of pathogens across multiple infection models, including both invasive and mucosal infection by Candida albicans, and lung infection by Aspergillus fumigatus and Cryptococcus neoformans.
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References
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Acknowledgement
This work was supported in part by NIH grants R21 DE023311 (A.P.M.), R56 AI111836 (A.P.M. and S.G.F.), R01 AI054928 (S.G.F.), and R01 DE017088 (S.G.F.).
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Xu, W., Solis, N.V., Filler, S.G., Mitchell, A.P. (2016). Pathogen Gene Expression Profiling During Infection Using a Nanostring nCounter Platform. In: Devaux, F. (eds) Yeast Functional Genomics. Methods in Molecular Biology, vol 1361. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3079-1_3
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DOI: https://doi.org/10.1007/978-1-4939-3079-1_3
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3078-4
Online ISBN: 978-1-4939-3079-1
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