Abstract
Genome-wide derivatives of the chromosome conformation capture (3C) technique are now well-established approaches to study the multiscale average organization of chromosomes from bacteria to mammals. However, the experimental parameters of the protocol have to be optimized for different species, and the downstream experimental products (i.e., pair-end sequences) are influenced by these parameters. Here, we describe a complete pipeline to generate 3C-seq libraries and compute chromosomal contact maps of yeast species.
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Acknowledgements
This research was supported by funding to R.K. from the European Research Council under the 7th Framework Program (FP7/2007-2013)/ERC grant agreement 260822. M.M. is the recipient of an Association pour la Recherche sur le Cancer fellowship 20100600373.
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Cournac, A., Marbouty, M., Mozziconacci, J., Koszul, R. (2016). Generation and Analysis of Chromosomal Contact Maps of Yeast Species. In: Devaux, F. (eds) Yeast Functional Genomics. Methods in Molecular Biology, vol 1361. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3079-1_13
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DOI: https://doi.org/10.1007/978-1-4939-3079-1_13
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3078-4
Online ISBN: 978-1-4939-3079-1
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