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Voltammetry in Behaving Animals

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Receptor and Ion Channel Detection in the Brain

Part of the book series: Neuromethods ((NM,volume 110))

Abstract

Fast-scan cyclic voltammetry (FSCV) is an electrochemical technique that provides rapid and reliable detection of key brain neurotransmitters (e.g., dopamine and other monoamines) with minimal tissue disruption. The technique can be applied to behaving animals, allowing for real-time monitoring of neurotransmitter release and uptake across a variety of behavioral situations. Changes in current due to the oxidation and reduction (redox reaction) of chemicals at the surface of the working electrode are recorded and later converted to concentration. Although the high spatial and temporal resolution of FSCV offers unprecedented access to the neurochemistry of brain function during behavior, a basic understanding of electrochemistry, instrumentation, and electrode performance is required to ensure accurate data analysis and interpretation. In this chapter, we introduce the basic concepts and methodology of FSCV and outline key experimental procedures.

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Author information

Authors and Affiliations

  1. Program in Neuroscience, Department of Psychological and Brain Sciences, Indiana University, Bloomington, IN, 47405, USA

    Kendra D. Bunner & George V. Rebec

Authors
  1. Kendra D. Bunner
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  2. George V. Rebec
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Corresponding author

Correspondence to Kendra D. Bunner .

Editor information

Editors and Affiliations

  1. Research Institute on Neurological Disabilities (IDINE), Department of Medical Sciences, School of Medicine, University of Castilla- La Mancha, Albacete, Spain

    Rafael Luján

  2. Pharmacology Unit, Department of Pathology and Experimental Therapeutics, School of Medicine, IDIBELL, University of Barcelona, Barcelona, Spain

    Francisco Ciruela

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Bunner, K.D., Rebec, G.V. (2016). Voltammetry in Behaving Animals. In: Luján, R., Ciruela, F. (eds) Receptor and Ion Channel Detection in the Brain. Neuromethods, vol 110. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3064-7_24

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  • DOI: https://doi.org/10.1007/978-1-4939-3064-7_24

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3063-0

  • Online ISBN: 978-1-4939-3064-7

  • eBook Packages: Springer Protocols

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