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Androgenesis in Solanaceae

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In Vitro Embryogenesis in Higher Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1359))

Abstract

The Solanaceae is one of the most important families for global agriculture. Among the different solanaceous species, tobacco (Nicotiana tabacum), potato (Solanum tuberosum), tomato (Solanum lycopersicum), eggplant (Solanum melongena), and pepper (Capsicum annuum) are five crops of outstanding importance worldwide. In these crops, maximum yields are produced by hybrid plants created by crossing pure (homozygous) lines with the desired traits. Pure lines may be produced by conventional breeding methods, which is time consuming and costly. Alternatively, it is possible to accelerate the production of pure lines by creating doubled haploid (DH) plants derived from (haploid) male gametophytes or their precursors (androgenesis). In this way, the different steps for the production of pure lines can be reduced to only one generation, which implies important time and cost savings. This and other advantages make androgenic DHs the choice in a number of important crops where any of the different experimental in vitro techniques (anther culture or isolated microspore culture) is well set up. The Solanaceae family is an excellent example of heterogeneity in terms of response to these techniques, including highly responding species such as tobacco, considered a model system, and tomato, one of the most recalcitrant species, where no reliable and reproducible methods are yet available. Interestingly, the first evidence of androgenesis, particularly through in vitro anther culture, was demonstrated in a solanaceous species, Datura innoxia. In this chapter, we report the state of the art of the research about androgenic DHs in Solanaceae, paying special attention to datura, tobacco, potato, tomato, eggplant, and pepper.

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Acknowledgments

Thanks are due to Dr. Satish C. Maheshwari for sharing with the author some bits of the history of Datura anther culture s, and to Mr. Edgar García for the acquisition of some of the pictures shown in this review. This work was supported by grant AGL2014-55177 from Spanish MINECO to J.M.S.S.

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  1. COMAV – Universitat Politècnica de València. CPI, Edificio 8E – Escalera I, Camino de Vera, 46022, Valencia, Spain

    Jose M. Seguí-Simarro

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  1. Jose M. Seguí-Simarro
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Correspondence to Jose M. Seguí-Simarro .

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  1. Dipartimento DEMETRA, Università degli Studi di Palermo, Palermo, Italy

    Maria Antonietta Germana

  2. National Research Council (CNR) IVALSA/Istituto per la Valorizzazione, Sesto Fiorentino, Florence, Italy

    Maurizio Lambardi

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Seguí-Simarro, J.M. (2016). Androgenesis in Solanaceae. In: Germana, M., Lambardi, M. (eds) In Vitro Embryogenesis in Higher Plants. Methods in Molecular Biology, vol 1359. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3061-6_9

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  • DOI: https://doi.org/10.1007/978-1-4939-3061-6_9

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