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Somatic Embryogenesis of Abies cephalonica Loud.

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In Vitro Embryogenesis in Higher Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1359))

Abstract

Greek fir (Abies cephalonica Loudon) belongs to the Mediterranean fir species and is widely distributed in the mountains of Central and Southern Greece. Considering a climatic scenario, infestation by pathogens or insects and fire episodes, it has been proposed that Mediterranean firs could be in danger in some parts of their present range but, on the other hand, could also replace other species in more northern zones with temperate humid climates (e.g., silver fir, Abies alba Mill.). As fir species are generally highly productive and therefore important for commercial forestry, they have traditionally been involved in conventional tree improvement programs. A lot of effort has been put into the development of vegetative propagation methods for firs, in order to rapidly gain the benefits of traditional breeding to be utilized in reforestation. The present paper provides up to date information on protocols for somatic embryogenesis (i.e., the most promising in vitro method for vegetative propagation) of Greek fir. Moreover, the protocols for cryopreservation and long-term storage of embryogenic material are described as well.

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Acknowledgments

The authors are grateful for the working facilities provided by University of Oulu, Genetics and Physiology Department.

Author information

Authors and Affiliations

  1. Department of Agriculture and Environmental Science, University of Udine, Via delle Scienze 91, Udine, 33100, Italy

    Jana Krajňáková

  2. Genetics and Physiology Department, University of Oulu, P.O. Box 3000, Oulu, 90014, Finland

    Jana Krajňáková & Hely Häggman

Authors
  1. Jana Krajňáková
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  2. Hely Häggman
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Corresponding author

Correspondence to Jana Krajňáková .

Editor information

Editors and Affiliations

  1. Dipartimento DEMETRA, Università degli Studi di Palermo, Palermo, Italy

    Maria Antonietta Germana

  2. National Research Council (CNR) IVALSA/Istituto per la Valorizzazione, Sesto Fiorentino, Florence, Italy

    Maurizio Lambardi

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Krajňáková, J., Häggman, H. (2016). Somatic Embryogenesis of Abies cephalonica Loud.. In: Germana, M., Lambardi, M. (eds) In Vitro Embryogenesis in Higher Plants. Methods in Molecular Biology, vol 1359. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3061-6_22

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  • DOI: https://doi.org/10.1007/978-1-4939-3061-6_22

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3060-9

  • Online ISBN: 978-1-4939-3061-6

  • eBook Packages: Springer Protocols

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