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Somatic Embryogenesis in Two Orchid Genera (Cymbidium, Dendrobium)

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In Vitro Embryogenesis in Higher Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1359))

Abstract

The protocorm-like body (PLB) is the de facto somatic embryo in orchids. Here we describe detailed protocols for two orchid genera (hybrid Cymbidium Twilight Moon ‘Day Light’ and Dendrobium ‘Jayakarta’, D. ‘Gradita 31’, and D. ‘Zahra FR 62’) for generating PLBs. These protocols will most likely have to be tweaked for different cultivars as the response of orchids in vitro tends to be dependent on genotype. In addition to primary somatic embryogenesis, secondary (or repetitive) somatic embryogenesis is also described for both genera. The use of thin cell layers as a sensitive tissue assay is outlined for hybrid Cymbidium while the protocol outlined is suitable for bioreactor culture of D. ‘Zahra FR 62’.

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Acknowledgements

The authors wish to thank Elsevier Ltd. for copyright permission to re-use photos in Fig. 1a, c, and f from [12]. The authors also thank Thammasat International Journal of Science and Technology for copyright permission to reuse the photo in Fig. 1b from [14].

Author information

Authors and Affiliations

  1. P.O. Box 7, Miki-cho Post Office, Ikenobe 3011-2, Kagawa-ken, 761-0799, Japan

    Jaime A. Teixeira da Silva

  2. Indonesian Ornamental Crops Research Institute (IOCRI), Jln. Raya Ciherang, Pacet-Cianjur, West Java, 43253, Indonesia

    Budi Winarto

Authors
  1. Jaime A. Teixeira da Silva
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  2. Budi Winarto
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Correspondence to Jaime A. Teixeira da Silva .

Editor information

Editors and Affiliations

  1. Dipartimento DEMETRA, Università degli Studi di Palermo, Palermo, Italy

    Maria Antonietta Germana

  2. National Research Council (CNR) IVALSA/Istituto per la Valorizzazione, Sesto Fiorentino, Florence, Italy

    Maurizio Lambardi

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Teixeira da Silva, J.A., Winarto, B. (2016). Somatic Embryogenesis in Two Orchid Genera (Cymbidium, Dendrobium). In: Germana, M., Lambardi, M. (eds) In Vitro Embryogenesis in Higher Plants. Methods in Molecular Biology, vol 1359. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3061-6_18

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  • DOI: https://doi.org/10.1007/978-1-4939-3061-6_18

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3060-9

  • Online ISBN: 978-1-4939-3061-6

  • eBook Packages: Springer Protocols

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