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Gene Expression in Mammalian Cells Using BacMam, a Modified Baculovirus System

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Baculovirus and Insect Cell Expression Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1350))

Abstract

BacMams are modified baculoviruses that contain mammalian expression cassettes for gene delivery and expression in mammalian cells. BacMams have become an integral part of the recombinant mammalian gene expression toolbox in research labs worldwide. Construction of transfer vectors is straightforward using basic molecular biology protocols. Virus generation is based on common methods used with the baculovirus insect cell expression system. BacMam transduction of mammalian cells requires minimal modifications to familiar cell culture methods. This chapter highlights the BacMam transfer vector pHTBV.

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Acknowledgement

The authors would like to thank Susan Merrihew and Linda Moore for the images in Fig. 5, and Tom Kost for helpful discussions.

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Authors and Affiliations

  1. Platform Technology and Science, GlaxoSmithKline, 1250 S. Collegeville Road, Collegeville, PA, 19426, USA

    James A. Fornwald, Quinn Lu & Robert S. Ames

  2. Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA

    Frederick M. Boyce

Authors
  1. James A. Fornwald
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  2. Quinn Lu
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  3. Frederick M. Boyce
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  4. Robert S. Ames
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Corresponding author

Correspondence to James A. Fornwald .

Editor information

Editors and Affiliations

  1. Engineering, University of Iowa Dept. Chemical & Biochemical, Iowa City, Iowa, USA

    David W. Murhammer

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Fornwald, J.A., Lu, Q., Boyce, F.M., Ames, R.S. (2016). Gene Expression in Mammalian Cells Using BacMam, a Modified Baculovirus System. In: Murhammer, D. (eds) Baculovirus and Insect Cell Expression Protocols. Methods in Molecular Biology, vol 1350. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3043-2_5

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  • DOI: https://doi.org/10.1007/978-1-4939-3043-2_5

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3042-5

  • Online ISBN: 978-1-4939-3043-2

  • eBook Packages: Springer Protocols

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