Abstract
Apoptosis is a physiological program of cell suicide conserved in invertebrates and vertebrates. Apoptosis is crucial to the normal development of organisms and in tissue homeostasis by promoting elimination of unwanted cells, including damaged or virus-infected cells. Due to the importance of programmed cell death for the survival of the organism, a tight regulation is exerted at various activation levels of the cell-death machinery. The utilization of the baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) to identify genes that inhibit the apoptotic process will be described using a transfection-based approach, illustrated by identification of the p49 gene.
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Acknowledgments
The author would like to thank Hadassah Rivkin for her excellent technical support. This work was supported by Israel Science Foundation Grant 426/99-3 and in part by Fogarty International Research Collaboration Award Grant TW01219.
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Chejanovsky, N. (2016). Using the Baculovirus/Insect Cell System to Study Apoptosis. In: Murhammer, D. (eds) Baculovirus and Insect Cell Expression Protocols. Methods in Molecular Biology, vol 1350. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3043-2_25
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DOI: https://doi.org/10.1007/978-1-4939-3043-2_25
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