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Production of Virus-Like Particles for Vaccination

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Baculovirus and Insect Cell Expression Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1350))

Abstract

The ability to make a large variety of virus-like particles (VLPs) has been successfully achieved in the baculovirus expression vector system (BEVS)/insect cell system. The production and scale-up of these particles, which are mostly sought as vaccine candidates, are currently being addressed. Furthermore, these VLPs are being investigated as delivery agents for use as therapeutics. The use of host insect cells allows mass production of VLPs in a proven scalable system.

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Acknowledgements

The authors would like to thank Johnny Montes, Emma Petiot, Parminder Chahal, Alice Bernier, and Julia Transfiguracion for their helpful advice and input throughout the writing of this chapter.

Author information

Authors and Affiliations

  1. Department of Chemical Engineering, Ecole Polytechnique de Montreal, 2500, Chemin de Polytechnique, Montreal, QC, Canada

    Christine M. Thompson

  2. National Research Council Canada, Montreal, QC, Canada

    Christine M. Thompson & Amine A. Kamen

  3. Department of Chemical Engineering, University of Waterloo, 200 University Ave W, Waterloo, ON, Canada, N2L 3G1

    Marc G. Aucoin

  4. Department of Bioengineering, McGill University, Montreal, QC, Canada

    Amine A. Kamen

Authors
  1. Christine M. Thompson
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  2. Marc G. Aucoin
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  3. Amine A. Kamen
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Corresponding author

Correspondence to Marc G. Aucoin .

Editor information

Editors and Affiliations

  1. Engineering, University of Iowa Dept. Chemical & Biochemical, Iowa City, Iowa, USA

    David W. Murhammer

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Thompson, C.M., Aucoin, M.G., Kamen, A.A. (2016). Production of Virus-Like Particles for Vaccination. In: Murhammer, D. (eds) Baculovirus and Insect Cell Expression Protocols. Methods in Molecular Biology, vol 1350. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3043-2_14

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  • DOI: https://doi.org/10.1007/978-1-4939-3043-2_14

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3042-5

  • Online ISBN: 978-1-4939-3043-2

  • eBook Packages: Springer Protocols

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