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High-Throughput Peptide Screening on a Bimodal Imprinting Chip Through MS-SPRi Integration

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Peptide Microarrays

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1352))

Abstract

Screening of high affinity and high specificity peptide probes towards various targets is important in the biomedical field while traditional peptide screening procedure is manual and tedious. Herein, a bimodal imprinting microarray system to embrace the whole peptide screening process is presented. Surface Plasmon Resonance imaging (SPRi) and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) are combined for both quantitative and qualitative identification of the peptide. The method provides a solution for high efficiency peptide screening.

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Acknowledgements

We acknowledge funding from the National Natural Science Foundation of China (21305023, 31270875), Beijing Municipal Natural Science Foundation (2144058), Project of Chinese Academy of Science (YZ201217), State Key Development Program for Basic Research of China grant (2011CB915502), and International Cooperation Project (0102010DFB33880).

Author information

Authors and Affiliations

  1. CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology of China, No.11. Beiyitiao Zhongguancun, Beijing, 100190, China

    Weizhi Wang, Qiaojun Fang & Zhiyuan Hu

Authors
  1. Weizhi Wang
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  2. Qiaojun Fang
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  3. Zhiyuan Hu
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Corresponding authors

Correspondence to Qiaojun Fang or Zhiyuan Hu .

Editor information

Editors and Affiliations

  1. Consiglio Nazionale delle Ricerche, Milano, Italy

    Marina Cretich

  2. Consiglio Nazionale delle Ricerche, Milano, Italy

    Marcella Chiari

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Wang, W., Fang, Q., Hu, Z. (2016). High-Throughput Peptide Screening on a Bimodal Imprinting Chip Through MS-SPRi Integration. In: Cretich, M., Chiari, M. (eds) Peptide Microarrays. Methods in Molecular Biology, vol 1352. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3037-1_9

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  • DOI: https://doi.org/10.1007/978-1-4939-3037-1_9

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3036-4

  • Online ISBN: 978-1-4939-3037-1

  • eBook Packages: Springer Protocols

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