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Peptide Microarrays pp 199–210Cite as

A Cell Microarray Format: A Peptide Release System Using a Photo-Cleavable Linker for Cell Toxicity and Cell Uptake Analysis

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1352))

Abstract

There has been increasing interest in the potential use of microarray technologies to perform systematic and high-throughput cell-based assays. We are currently focused on developing more practical array formats and detection methods that will enable researchers to conduct more detailed analyses in cell microarray studies. In this chapter, we describe the construction of a novel peptide-array format system for analyzing cellular toxicity and cellular uptake. In this system, a peptide is immobilized at the bottom of a conventional 96-well plate using a photo-cleavable linker. The peptide can then be released from the bottom by irradiating the desired wells with UV light, thus allowing the cytotoxicity or cellular uptake of the peptide to be monitored. This system will facilitate the realization of high-throughput cell arrays for cellomics analyses and cell-based phenotypic drug screens.

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Acknowledgments

We thank Mr. T. Kakiyama and Mr. T. Kikuchi (Tokyo Institute of Technology, Yokohama) for valuable discussions and generous support. This study was supported in part by grants from JSPS KAKENHI and NAGASE Science Technology Foundation. K.U. is also grateful to the JSPS KAKENHI Grant Number 26750375 from MEXT and the Grant-in-Aid for Encouragement of Young Scientists from Nakatani Foundation. K.-Y. T. acknowledges the Ryukoku University Science and Technology Fund.

Author information

Authors and Affiliations

  1. FIRST (Faculty of Frontiers of Innovative Research in Science and Technology), Konan University, 7-1-20 Minatojima-minamimachi, Chuo-ku, Kobe, 650-0047, Japan

    Kenji Usui

  2. Department of Materials Chemistry, Ryukoku University, Seta-Oe, Otsu, 520-2194, Japan

    Kin-ya Tomizaki

  3. Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta-cho 4259 B-40, Midori-ku, Yokohama, 226-8501, Japan

    Hisakazu Mihara

Authors
  1. Kenji Usui
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  2. Kin-ya Tomizaki
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  3. Hisakazu Mihara
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Corresponding author

Correspondence to Kenji Usui .

Editor information

Editors and Affiliations

  1. Consiglio Nazionale delle Ricerche, Milano, Italy

    Marina Cretich

  2. Consiglio Nazionale delle Ricerche, Milano, Italy

    Marcella Chiari

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Usui, K., Tomizaki, Ky., Mihara, H. (2016). A Cell Microarray Format: A Peptide Release System Using a Photo-Cleavable Linker for Cell Toxicity and Cell Uptake Analysis. In: Cretich, M., Chiari, M. (eds) Peptide Microarrays. Methods in Molecular Biology, vol 1352. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3037-1_15

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  • DOI: https://doi.org/10.1007/978-1-4939-3037-1_15

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3036-4

  • Online ISBN: 978-1-4939-3037-1

  • eBook Packages: Springer Protocols

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