Abstract
A new methodology for the fabrication of an high-performance peptide microarray is reported, combining the higher sensitivity of a layered Si–SiO2 substrate with the oriented immobilization of peptides using a N,N-dimethylacrylamide-based polymeric coating that contains alkyne monomers as functional groups. This clickable polymer allows the oriented attachment of azido-modified peptides via a copper-mediated azide/alkyne cycloaddition. A similar coating that does not contain the alkyne functionality has been used as comparison, to demonstrate the importance of a proper orientation for facilitating the probe recognition and interaction with the target antibody.
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Sola, L., Gori, A., Cretich, M., Finetti, C., Zilio, C., Chiari, M. (2016). Clickable Polymeric Coating for Oriented Peptide Immobilization. In: Cretich, M., Chiari, M. (eds) Peptide Microarrays. Methods in Molecular Biology, vol 1352. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3037-1_13
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DOI: https://doi.org/10.1007/978-1-4939-3037-1_13
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3036-4
Online ISBN: 978-1-4939-3037-1
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