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Production and Screening of Monoclonal Peptide Antibodies

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Peptide Antibodies

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1348))

Abstract

Hybridoma technology is a remarkable and indispensable tool for generating high-quality monoclonal antibodies. Hybridoma-derived monoclonal antibodies not only serve as powerful research and diagnostic reagents, but have also emerged as the most rapidly expanding class of therapeutic biologicals. In this chapter, an overview of hybridoma technology and the laboratory procedures used routinely for hybridoma production and antibody screening are presented, including characterization of peptide antibodies.

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Authors and Affiliations

  1. Department of Autoimmunology and Biomarkers, Statens Serum Institut, Artillerivej 5, 2300, Copenhagen, Denmark

    Nicole Hartwig Trier, Anne Mortensen, Annette Schiolborg & Tina Friis

Authors
  1. Nicole Hartwig Trier
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  2. Anne Mortensen
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  3. Annette Schiolborg
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  4. Tina Friis
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Corresponding author

Correspondence to Tina Friis .

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Editors and Affiliations

  1. Statens Serum Institut, Copenhagen, Denmark

    Gunnar Houen

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Trier, N.H., Mortensen, A., Schiolborg, A., Friis, T. (2015). Production and Screening of Monoclonal Peptide Antibodies. In: Houen, G. (eds) Peptide Antibodies. Methods in Molecular Biology, vol 1348. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2999-3_12

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  • DOI: https://doi.org/10.1007/978-1-4939-2999-3_12

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2998-6

  • Online ISBN: 978-1-4939-2999-3

  • eBook Packages: Springer Protocols

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