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Posttranslational Modifications of TGF-β Receptors

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TGF-β Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1344))

Abstract

TGF-β is a prototype of the TGF-β cytokine superfamily and exerts multiple regulatory effects on cell activities. It signals through two types of membrane-bound serine/threonine kinase receptors. Upon TGF-β binding, the type II receptor TβRII recruits the type I receptor TβRI and form a functional heterocomplex. TβRII trans-phosphorylates the GS region of TβRI, thus triggering its kinase activity. Activated TβRI proceeds to activate downstream Smad2/3. Signal intensity and duration through the availability, activity and destiny of TGF-β receptors are finely controlled by multiple posttranslational modifications such as phosphorylation, ubiquitination, and neddylation. This chapter introduces methods for examination of these modifications of TGF-β receptors.

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Acknowledgement

We thank Dr. Fei Huang for critical reading of the manuscript.

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Authors and Affiliations

  1. State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, 100084, China

    Xiaohua Yan & Ye-Guang Chen

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  1. Xiaohua Yan
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  2. Ye-Guang Chen
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Corresponding author

Correspondence to Ye-Guang Chen .

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Editors and Affiliations

  1. Departments of Surgery and Molecular & Cellular Biology, Baylor College of Medicine, Houston, Texas, USA

    Xin-Hua Feng

  2. Life Sciences Institute, Zhejiang University, Hangzhou, Zhejiang Proviince, China

    Pinglong Xu

  3. Department of Surgery, Baylor College of Medicine, Houston, Texas, USA

    Xia Lin

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Yan, X., Chen, YG. (2016). Posttranslational Modifications of TGF-β Receptors. In: Feng, XH., Xu, P., Lin, X. (eds) TGF-β Signaling. Methods in Molecular Biology, vol 1344. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2966-5_3

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  • DOI: https://doi.org/10.1007/978-1-4939-2966-5_3

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2965-8

  • Online ISBN: 978-1-4939-2966-5

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