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Isolation and Manipulation of Adipogenic Cells to Assess TGF-β Superfamily Functions

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TGF-β Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1344))

Abstract

A variety of TGF-β superfamily members affect adipocyte differentiation and function with consequential effects on energy metabolism. There has been a growing interest in this area because of the apparent influence of the BMP subgroup on brown adipose characteristics and potential application to the treatment of human obesity. In this chapter we describe methods that are useful in allowing one to assess the roles of specific members of the superfamily on adipocyte differentiation and mature adipocyte function, including the isolation and differentiation of mouse embryo fibroblasts (MEFs) to examine cell autonomous effects and the efficient transfection of two commonly used (but difficult to transfect) adipogenic cell lines, C3H/10T1/2 and 3T3-L1.

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Author information

Authors and Affiliations

  1. Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA

    Maria Namwanje & Chester W. Brown

  2. Department of Pediatrics, Baylor College of Medicine, Houston, TX, USA

    Juan C. Bournat & Chester W. Brown

  3. Texas Children’s Hospital, Houston, TX, USA

    Chester W. Brown

Authors
  1. Maria Namwanje
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  2. Juan C. Bournat
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  3. Chester W. Brown
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Corresponding author

Correspondence to Chester W. Brown .

Editor information

Editors and Affiliations

  1. Departments of Surgery and Molecular & Cellular Biology, Baylor College of Medicine, Houston, Texas, USA

    Xin-Hua Feng

  2. Life Sciences Institute, Zhejiang University, Hangzhou, Zhejiang Proviince, China

    Pinglong Xu

  3. Department of Surgery, Baylor College of Medicine, Houston, Texas, USA

    Xia Lin

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Namwanje, M., Bournat, J.C., Brown, C.W. (2016). Isolation and Manipulation of Adipogenic Cells to Assess TGF-β Superfamily Functions. In: Feng, XH., Xu, P., Lin, X. (eds) TGF-β Signaling. Methods in Molecular Biology, vol 1344. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2966-5_12

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  • DOI: https://doi.org/10.1007/978-1-4939-2966-5_12

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2965-8

  • Online ISBN: 978-1-4939-2966-5

  • eBook Packages: Springer Protocols

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