Abstract
The rapid development of deep sequencing technologies over the last few years and concomitant increases in sequencing depth and cost efficiencies have opened the door to a ever-widening range of applications in biology—from whole-genome sequencing, to ChIP-seq analysis, epigenomic and RNA transcriptome surveys. Here we describe the application of deep sequencing to the discovery of novel microRNAs and characterization of their differential expression during adulthood in Caenorhabditis elegans.
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Acknowledgement
Some C. elegans strains were provided by the CGC, which is funded by NIH Office of Research Infrastructure Programs (P40 OD010440). We thank Dr. Giovanni Stefani and Dr. Masaomi Kato for help with methods. A.d.L. was supported by a National Research Service Award Postdoctoral Fellowship from the National Institutes of Health (NIH; 1F32AG030851). F.J.S. was supported by a Breakthroughs in Gerontology grant from the American Federation for Aging Research, the Ellison Medical Foundation, and the NIH (AG033921).
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de Lencastre, A., Slack, F. (2015). Discovery of Novel microRNAs in Aging Caenorhabditis elegans . In: Shaw, A. (eds) Immunosenescence. Methods in Molecular Biology, vol 1343. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2963-4_18
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DOI: https://doi.org/10.1007/978-1-4939-2963-4_18
Publisher Name: Humana Press, New York, NY
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