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Proteomic Analysis of Engineered Cartilage

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Cartilage Tissue Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1340))

Abstract

Tissue engineering holds promise for the treatment of damaged and diseased tissues, especially for those tissues that do not undergo repair and regeneration readily in situ. Many techniques are available for cell and tissue culturing and differentiation of chondrocytes using a variety of cell types, differentiation methods, and scaffolds. In each case, it is critical to demonstrate the cellular phenotype and tissue composition, with particular attention to the extracellular matrix molecules that play a structural role and that contribute to the mechanical properties of the resulting tissue construct. Mass spectrometry provides an ideal analytical method with which to characterize the full spectrum of proteins produced by tissue-engineered cartilage. Using normal cartilage tissue as a standard, tissue-engineered cartilage can be optimized according to the entire proteome. Proteomic analysis is a complementary approach to biochemical, immunohistochemical, and mechanical testing of cartilage constructs. Proteomics is applicable as an analysis approach to most cartilage constructs generated from a variety of cellular sources including primary chondrocytes, mesenchymal stem cells from bone marrow, adipose tissue, induced pluripotent stem cells, and embryonic stem cells. Additionally, proteomics can be used to optimize novel scaffolds and bioreactor applications, yielding cartilage tissue with the proteomic profile of natural cartilage.

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Acknowledgements

Authors wish to thank Barbara Jibben for technical support. This work was supported by Institutional Development Awards (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under grant numbers P20GM103408 and P20GM109095, NNX10AN29A from NASA, and the Boise State University Biomolecular Research Center.

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Authors and Affiliations

  1. Department of Biological Sciences, Biomolecular Research Center, Boise State University, Boise, ID, USA

    Xinzhu Pu

  2. Department of Biological Sciences, Biomolecular Research Center, Boise State University, 1910 University Drive, Mail Stop 1511, Boise, ID, USA

    Julia Thom Oxford

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  1. Xinzhu Pu
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  2. Julia Thom Oxford
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Corresponding author

Correspondence to Julia Thom Oxford .

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Editors and Affiliations

  1. Swinburne University of Technology, Faculty of Science, Engineering and Technology, Hawthorn, Melbourne, Victoria, Australia

    Pauline M. Doran

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Pu, X., Oxford, J.T. (2015). Proteomic Analysis of Engineered Cartilage. In: Doran, P. (eds) Cartilage Tissue Engineering. Methods in Molecular Biology, vol 1340. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2938-2_19

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  • DOI: https://doi.org/10.1007/978-1-4939-2938-2_19

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2937-5

  • Online ISBN: 978-1-4939-2938-2

  • eBook Packages: Springer Protocols

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