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Protein Arginylation pp 49–58Cite as

Assaying the Posttranslational Arginylation of Proteins in Cultured Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1337))

Abstract

To evaluate the posttranslational arginylation of proteins in vivo, we describe a protocol for studying the 14C-Arg incorporation into proteins of cells in culture. The conditions determined for this particular modification contemplate both the biochemical requirements of the enzyme ATE1 and the adjustments that allowed the discrimination between posttranslational arginylation of proteins and de novo synthesis. These conditions are applicable for different cell lines or primary cultures, representing an optimal procedure for the identification and the validation of putative ATE1 substrates.

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Acknowledgments

M.R.G. and M.E.H. are career investigators from CONICET.

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Authors and Affiliations

  1. Departamento de Química Biológica, Facultad de Ciencias Químicas, Centro de Investigaciones en Química Biológica de Córdoba, CIQUIBIC, (UNC-CONICET), Universidad Nacional de Córdoba (X5000HUA), Córdoba, Argentina

    Mauricio R. Galiano & Marta E. Hallak

Authors
  1. Mauricio R. Galiano
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  2. Marta E. Hallak
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Corresponding author

Correspondence to Marta E. Hallak .

Editor information

Editors and Affiliations

  1. Department of Animal Biology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, Pennsylvania, USA

    Anna S. Kashina

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Galiano, M.R., Hallak, M.E. (2015). Assaying the Posttranslational Arginylation of Proteins in Cultured Cells. In: Kashina, A. (eds) Protein Arginylation. Methods in Molecular Biology, vol 1337. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2935-1_7

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  • DOI: https://doi.org/10.1007/978-1-4939-2935-1_7

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2934-4

  • Online ISBN: 978-1-4939-2935-1

  • eBook Packages: Springer Protocols

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