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TALENs pp 89–97Cite as

A New Approach to Dissect Nuclear Organization: TALE-Mediated Genome Visualization (TGV)

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1338))

Abstract

Spatiotemporal organization of chromatin within the nucleus has so far remained elusive. Live visualization of nuclear remodeling could be a promising approach to understand its functional relevance in genome functions and mechanisms regulating genome architecture. Recent technological advances in live imaging of chromosomes begun to explore the biological roles of the movement of the chromatin within the nucleus. Here I describe a new technique, called TALE-mediated genome visualization (TGV), which allows us to visualize endogenous repetitive sequence including centromeric, pericentromeric, and telomeric repeats in living cells.

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Author information

Authors and Affiliations

  1. Okazaki Institute for integrative bioscience, Okazaki, 444-8787, Japan

    Yusuke Miyanari

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  1. Yusuke Miyanari
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Correspondence to Yusuke Miyanari .

Editor information

Editors and Affiliations

  1. Max-Delbrück-Center for Molecular Medicine, Berlin, Germany

    Ralf Kühn

  2. Institute of Developmental Genetics, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany

    Wolfgang Wurst

  3. Institute of Developmental Genetics, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany

    Benedikt Wefers

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Miyanari, Y. (2016). A New Approach to Dissect Nuclear Organization: TALE-Mediated Genome Visualization (TGV). In: Kühn, R., Wurst, W., Wefers, B. (eds) TALENs. Methods in Molecular Biology, vol 1338. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2932-0_8

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  • DOI: https://doi.org/10.1007/978-1-4939-2932-0_8

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2931-3

  • Online ISBN: 978-1-4939-2932-0

  • eBook Packages: Springer Protocols

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