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Online Tools for TALEN Design

  • Protocol
TALENs

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1338))

Abstract

Transcription activator-like effector nucleases (TALENs) can be exquisitely specific and highly effective genome editing reagents. Specificity and efficacy depend however on good design for minimal off-targeting and strong binding. Several online tools are accessible to aid in this process. Here, we tabulate those tools, noting their functions and key features.

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Acknowledgement

Research on TAL effectors in our laboratory is supported by grants from the National Science Foundation (IOS 1238189) and the National Institutes of Health (R01 GM098861).

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Authors and Affiliations

  1. Plant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell University, 334 Plant Science, Ithaca, NY, 14853, USA

    Adam J. Bogdanove & Nicholas J. Booher

Authors
  1. Adam J. Bogdanove
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  2. Nicholas J. Booher
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Corresponding author

Correspondence to Adam J. Bogdanove .

Editor information

Editors and Affiliations

  1. Max-Delbrück-Center for Molecular Medicine, Berlin, Germany

    Ralf Kühn

  2. Institute of Developmental Genetics, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany

    Wolfgang Wurst

  3. Institute of Developmental Genetics, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany

    Benedikt Wefers

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© 2016 Springer Science+Business Media New York

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Bogdanove, A.J., Booher, N.J. (2016). Online Tools for TALEN Design. In: Kühn, R., Wurst, W., Wefers, B. (eds) TALENs. Methods in Molecular Biology, vol 1338. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2932-0_4

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  • DOI: https://doi.org/10.1007/978-1-4939-2932-0_4

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2931-3

  • Online ISBN: 978-1-4939-2932-0

  • eBook Packages: Springer Protocols

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