Abstract
Gene engineering for generating targeted mouse mutants is a key technology for biomedical research. Using TALENs as sequence-specific nucleases to induce targeted double-strand breaks, the mouse genome can be directly modified in zygotes in a single step without the need for embryonic stem cells. By embryo microinjection of TALEN mRNAs and targeting vectors, knockout and knock-in alleles can be generated fast and efficiently. In this chapter we provide protocols for the application of TALENs in mouse zygotes.
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Acknowledgements
This work was supported by the European Union within the EUCOMM project (LSHG-CT-2005-018931, to W.W.), by the German Ministry of Education and Research within the TAL-CUT (03 V0261) and DIGTOP project (01GS0858) (to W.W. and R.K.) of the VIP and NGFN-Plus program.
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Wefers, B., Brandl, C., Ortiz, O., Wurst, W., Kühn, R. (2016). Genome Editing in Mice Using TALE Nucleases. In: Kühn, R., Wurst, W., Wefers, B. (eds) TALENs. Methods in Molecular Biology, vol 1338. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2932-0_17
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DOI: https://doi.org/10.1007/978-1-4939-2932-0_17
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