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Targeted Mutagenesis in Bombyx mori Using TALENs

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TALENs

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1338))

Abstract

Bombyx mori is a valuable model organism of high economic importance. Its genome sequence is available, as well as basic genetic and molecular genetic tools and markers. The introduction of genome editing methods based on engineered nucleases enables precise manipulations with genomic DNA, including targeted DNA deletions, insertions, or replacements in the genome allowing gene analysis and various applications. We describe here the use of TALENs which have a simple modular design of their DNA-binding domains, are easy to prepare and proved to be efficient in targeting of a wide range of cleavage sites. Our procedure often allows the production of individuals carrying homozygous mutations as early as in the G1 generation.

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Acknowledgements

This research was supported by KAKENHI Grant Number 23580083 from Japan Society for the Promotion of Science and grant P305/10/2406 from the Grant Agency of the Czech Republic. We acknowledge the use of research infrastructure that has received funding from the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement no 316304. We would like to thank Dr. František Sehnal for critical reading of the manuscript.

Author information

Authors and Affiliations

  1. National Institute of Agrobiological Sciences, 1-2 Owashi, Tsukuba, Ibaraki, 305-8634, Japan

    Yoko Takasu & Toshiki Tamura

  2. Department of Biological Sciences, University of Rhode Island, 120 Flagg Road, Kingston, RI, 02881-0816, USA

    Marian Goldsmith

  3. Biology Centre, Czech Academy of Sciences, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic

    Michal Zurovec

Authors
  1. Yoko Takasu
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  2. Toshiki Tamura
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  3. Marian Goldsmith
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  4. Michal Zurovec
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Corresponding authors

Correspondence to Toshiki Tamura or Michal Zurovec .

Editor information

Editors and Affiliations

  1. Max-Delbrück-Center for Molecular Medicine, Berlin, Germany

    Ralf Kühn

  2. Institute of Developmental Genetics, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany

    Wolfgang Wurst

  3. Institute of Developmental Genetics, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany

    Benedikt Wefers

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Takasu, Y., Tamura, T., Goldsmith, M., Zurovec, M. (2016). Targeted Mutagenesis in Bombyx mori Using TALENs. In: Kühn, R., Wurst, W., Wefers, B. (eds) TALENs. Methods in Molecular Biology, vol 1338. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2932-0_11

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  • DOI: https://doi.org/10.1007/978-1-4939-2932-0_11

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2931-3

  • Online ISBN: 978-1-4939-2932-0

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