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Intravital Microscopy for Atherosclerosis Research

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Methods in Mouse Atherosclerosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1339))

Abstract

Recruitment of leukocytes into arteries is a hallmark event throughout all stages of atherosclerosis and hence stands out as a primary therapeutic target. To understand the molecular mechanisms of arterial leukocyte subset infiltration, real-time visualization of recruitment processes of leukocyte subsets at high resolution is a prerequisite. In this review we provide a balanced overview of optical imaging modalities in the more commonly used experimental models for atherosclerosis (e.g., mouse models) allowing for in vivo display of recruitment processes in large arteries and further detail strategies to overcome hurdles inherent to arterial imaging. We further provide a synopsis of techniques allowing for non-toxic, photostable labeling of target structures. Finally, we deliver a short summary of ongoing developments including the emergence of novel labeling approaches, the use of superresolution microscopy, and the potentials of opto-acoustic microscopy and intravascular 2-dimensional near-infrared fluorescence microscopy.

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Acknowledgements

The work of the authors’ is supported by the DFG (SO876/3-1, SO876/6-1, SFB914 TP B08, SFB1123 TPs A06, B05, Z01, INST409/97-1), the Else Kröner Fresenius Stiftung, the NWO (VIDI project 91712303), the LMUexcellence, and the FöFoLe program of the LMU Munich.

Conflict of interest

None declared.

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Authors and Affiliations

  1. Institute for Cardiovascular Prevention, Ludwig-Maximilians-University Munich, Pettenkoferstr. 9, 80336, Munich, Germany

    Remco T. A. Megens & Oliver Soehnlein

  2. Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands

    Remco T. A. Megens

  3. Department of Pathology, Academic Medical Center, Amsterdam University, Amsterdam, The Netherlands

    Oliver Soehnlein

  4. DZHK (German Centre for Cardiovascular Research), partner site Munich Heart Alliance, Munich, Germany

    Oliver Soehnlein

Authors
  1. Remco T. A. Megens
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  2. Oliver Soehnlein
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Correspondence to Remco T. A. Megens or Oliver Soehnlein .

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Editors and Affiliations

  1. Laboratory of Molecular and Genetic Cardiovascular Pathophysiology, Centro Nacional de Investigaciones Cardiovascular Carlos III (CNIC), Madrid, Madrid, Spain

    Vicente Andrés

  2. Laboratory of Molecular and Genetic Cardiovascular Pathophysiology, Centro Nacional de Investigaciones Cardiovascular Carlos III (CNIC), Madrid, Madrid, Spain

    Beatriz Dorado

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Megens, R.T.A., Soehnlein, O. (2015). Intravital Microscopy for Atherosclerosis Research. In: Andrés, V., Dorado, B. (eds) Methods in Mouse Atherosclerosis. Methods in Molecular Biology, vol 1339. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2929-0_3

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  • DOI: https://doi.org/10.1007/978-1-4939-2929-0_3

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2928-3

  • Online ISBN: 978-1-4939-2929-0

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