Abstract
Small arteries play an important role in regulation of peripheral resistance and organ perfusion. Here we describe a series of methods allowing measurements in pressurized segments of small arteries from the systemic and coronary circulation of mice as well as other species. The pressure myography techniques described include measurements of wall structure, wall stress, strain, and myogenic tone. The pressurized perfused small arteries also allow evaluation of responses to increases in pressure, flow, and drugs, where the main readout is changes in vascular diameter.
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Acknowledgment
Ulf Simonsen is supported by the Danish Heart Foundation and is part of LiPhos (Living Photonics: Monitoring light propagation through cells), which is funded by the EC Seventh Framework Programme.
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Schjørring, O.L., Carlsson, R., Simonsen, U. (2015). Pressure Myography to Study the Function and Structure of Isolated Small Arteries. In: Andrés, V., Dorado, B. (eds) Methods in Mouse Atherosclerosis. Methods in Molecular Biology, vol 1339. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2929-0_19
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DOI: https://doi.org/10.1007/978-1-4939-2929-0_19
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2928-3
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