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Cell Synchronization Techniques to Study the Action of CDK Inhibitors

  • Beatriz Pérez-Benavente
  • Rosa FarràsEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1336)

Abstract

Cell synchronization techniques have been used for the studies of mechanisms involved in cell cycle regulation. Synchronization involves the enrichment of subpopulations of cells in specific stages of the cell cycle. These subpopulations are then used to study regulatory mechanisms of the cell cycle such as DNA synthesis, gene expression, protein synthesis, protein phosphorylation, protein degradation, and development of new drugs (e.g., CDK inhibitors). Here, we describe several protocols for synchronization of cells from different phases of the cell cycle. We also describe protocols for determining cell viability and mitotic index and for validating the synchrony of the cells by flow cytometry.

Key words

Cell cycle DNA content Flow cytometry Mitotic index Synchronization 

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Copyright information

© Springer Science+Business Media, LLC 2016

Authors and Affiliations

  1. 1.Oncogenic Signalling LaboratoryCentro de Investigación Príncipe Felipe de ValenciaValenciaSpain
  2. 2.Biochemistry and Molecular Biology DepartmentUniversidad de ValenciaValenciaSpain

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