Expression and Purification of Recombinant Cyclins and CDKs for Activity Evaluation

  • Edurne Gallastegui
  • Oriol BachsEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1336)


Cyclin-dependent kinases (Cdks) belong to a family of key regulators of cell division cycle and transcription. Their activity is mainly regulated by association with regulatory subunits named cyclins but their activities are also regulated by phosphorylation, acetylation, and the association with specific inhibitory proteins (CKIs). The activity of different Cdks is deregulated in many different type of tumors, and thus, Cdks are considered targets for antitumoral therapy. For large screenings of inhibitors the use of purified recombinant Cdks and cyclins is recommended. We report here the current methods to determine their in vitro activity for large screenings of inhibitors.

Key words

Cdk1 Cdk2 Cyclin A Cyclin B Kinase assay 


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Copyright information

© Springer Science+Business Media, LLC 2016

Authors and Affiliations

  1. 1.Department of Cell Biology, Immunology and NeurosciencesUniversity of BarcelonaBarcelonaSpain
  2. 2.Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS)BarcelonaSpain

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