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Evaluating Chemical CDK Inhibitors as Cell Death Inducers

  • Hiroshi HiraiEmail author
  • Yoko Nakatsuru
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Part of the Methods in Molecular Biology book series (MIMB, volume 1336)

Abstract

The cell cycle of eukaryotic cells is regulated by a family of protein kinases called cyclin-dependent kinases (Cdks). We have reported the identification and biological characterization of a highly potent, small-molecule pan-Cdk inhibitor, which inhibited Cdk1, 2, 4, 5, 6, and 9 with equal potency in the nM range. This compound inhibited multiple events in the cell cycle and induced cell death in human cancer cell lines as well as in peripheral blood or purified resting lymphocytes ex vivo. We describe the materials and methods to determine antitumor efficacy in vivo xenograft models. Pharmacodynamic marker assays that have been performed using tumors and normal tissues are explained. Moreover, we briefly describe methods for determining the effects of chemical Cdk inhibitors on peripheral blood cells or lymphocytes ex vivo.

Key words

Antitumor efficacy Cdk inhibitor Immunohistochemistry (IHC) assay In vivo xenograft model In vivo pharmacodynamics (PD) assay Lymphocytes Peripheral blood cells 

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Copyright information

© Springer Science+Business Media, LLC 2016

Authors and Affiliations

  1. 1.Taiho Pharmaceutical Co. Ltd.Tsukuba Research CenterTsukubaJapan

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