Immunoprecipitation of Cdk–Cyclin Complexes for Determination of Kinase Activity
Cyclin-dependent kinases (Cdks) belong to a family of key regulators of cell division cycle and transcription. The activity of some of them is deregulated in tumor cells and to find specific inhibitors is an important goal to be achieved. We report here the current methods to determine their in vitro activity in order to facilitate the identification of specific inhibitors. Mainly, the activity can be determined by using immunoprecipitates from cell samples with antibodies against specific Cdks as a source of the enzymes.
Key wordsCdk2 Cdk4 Cdk6 Cdk1 Cyclin A Cyclin B Cyclin D Kinase assay