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Characterization of Carbohydrate Vaccines by NMR Spectroscopy

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Carbohydrate-Based Vaccines

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1331))

Abstract

Physicochemical techniques are a powerful tool for the structural characterization of carbohydrate-based vaccines. High-field Nuclear Magnetic Resonance (NMR) spectroscopy has been established as an extremely useful and robust method for tracking the industrial manufacturing process of these vaccines from polysaccharide bulk antigen through to the final formulation. Here, we describe the use of proton NMR for structural identity and conformity testing of carbohydrate-based vaccines.

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Acknowledgements

We thank Dr. Chris Jones (Laboratory for Molecular Structure, National Institute for Biological Standards and Control) for years of useful discussions on NMR and vaccines. One of us (N.R.) would like to thank PATH and all the vaccine manufacturers who have made available samples of polysaccharides and glycoconjugate vaccines.

Author information

Authors and Affiliations

  1. Research, GSK Vaccines, Via Fiorentina 1, 53100, Siena, Italy

    Francesco Berti

  2. Department of Chemistry, University of Cape Town, Rondebosch, 7701, South Africa

    Neil Ravenscroft

Authors
  1. Francesco Berti
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  2. Neil Ravenscroft
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Corresponding author

Correspondence to Francesco Berti .

Editor information

Editors and Affiliations

  1. Research Center for Emerging Infections and Zoonoses, University of Veterinary Medicine, Hannover, Germany

    Bernd Lepenies

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Berti, F., Ravenscroft, N. (2015). Characterization of Carbohydrate Vaccines by NMR Spectroscopy. In: Lepenies, B. (eds) Carbohydrate-Based Vaccines. Methods in Molecular Biology, vol 1331. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2874-3_12

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  • DOI: https://doi.org/10.1007/978-1-4939-2874-3_12

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2873-6

  • Online ISBN: 978-1-4939-2874-3

  • eBook Packages: Springer Protocols

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