Abstract
Modern vaccines such as recombinant proteins or nucleic acids are usually of pure origin, enhancing their tolerability and overall safety. However, this purity often renders them less immunogenic, creating the need for potent adjuvants. Carbohydrates are promising candidates to fulfill this role as they enable direct targeting of dendritic cells and modulation of adaptive immunity. C-type lectin receptors (CLRs) comprise a major group of carbohydrate binding receptors. As they are predominantly expressed by cells of innate immunity, CLR targeting can enhance or dampen early stages of cytokine secretion and antigen presentation, thus modulate the activation and differentiation of T cells. Here, we provide a protocol for the identification of novel CLR ligands by glycan array using recombinant CLR-Fc chimeras followed by the covalent conjugation of carbohydrate CLR ligands to the model antigen ovalbumin (OVA). The resulting glycoconjugates are subsequently used to evaluate T cell activation in vitro and immunomodulation in vivo.
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Acknowledgements
This work was supported by the Max Planck Society. We further acknowledge funding by the German Federal Ministry of Education and Research (Fkz. 0315446 to B. L.) and the SFB 765 (to B.L.).
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Johannssen, T., Lepenies, B. (2015). Identification and Characterization of Carbohydrate-Based Adjuvants. In: Lepenies, B. (eds) Carbohydrate-Based Vaccines. Methods in Molecular Biology, vol 1331. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2874-3_11
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DOI: https://doi.org/10.1007/978-1-4939-2874-3_11
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2873-6
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