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The β-Barrel Assembly Machinery Complex

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The BAM Complex

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1329))

Abstract

The outer membranes of gram-negative bacteria contain integral membrane proteins, most of which are of β-barrel structure, and critical for bacterial survival. These β-barrel proteins rely on the β-barrel assembly machinery (BAM) complex for their integration into the outer membrane as folded species. The central and essential subunit of the BAM complex, BamA, is a β-barrel protein conserved in all gram-negative bacteria and also found in eukaryotic organelles derived from bacterial endosymbionts. In Escherichia coli, BamA docks with four peripheral lipoproteins, BamB, BamC, BamD and BamE, partner subunits that add to the function of the BAM complex in outer membrane protein biogenesis. By way of introduction to this volume, we provide an overview of the work that has illuminated the mechanism by which the BAM complex drives β-barrel assembly. The protocols and methodologies associated with these studies as well as the challenges encountered and their elegant solutions are discussed in subsequent chapters.

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Acknowledgements

We thank Chaille Webb, Victoria Hewitt, and Christopher Stubenrauch for constructive comments on the manuscript. We acknowledge support from the Australian Research Council (ARC) for research funding through the Super Science Fellowship grant FS110200015 (to T.L.) and NHMRC Program Grant 606788 (to T.L.). D.L.L. is an ARC Super Science Fellow, M.J.B. is an NHMRC Biomedical Research Fellow and T.L. is an ARC Australian Laureate Fellow.

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Authors and Affiliations

  1. Research School of Biology, Australian National University, Canberra, ACT, 0200, Australia

    Denisse L. Leyton

  2. Department of Microbiology, Monash University, Building 77, 23 Innovation Walk, Clayton Campus, Melbourne, VIC, 3800, Australia

    Matthew J. Belousoff & Trevor Lithgow

Authors
  1. Denisse L. Leyton
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  2. Matthew J. Belousoff
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  3. Trevor Lithgow
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Correspondence to Denisse L. Leyton or Trevor Lithgow .

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Editors and Affiliations

  1. National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Laboratory of Molecular Biology, , Bethesda, Maryland, USA

    Susan Buchanan

  2. Department of Biological Sciences,, Markey Center for Structural Biology, Purdue University, West Lafayette, Indiana, USA

    Nicholas Noinaj

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Leyton, D.L., Belousoff, M.J., Lithgow, T. (2015). The β-Barrel Assembly Machinery Complex. In: Buchanan, S., Noinaj, N. (eds) The BAM Complex. Methods in Molecular Biology, vol 1329. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2871-2_1

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  • DOI: https://doi.org/10.1007/978-1-4939-2871-2_1

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