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Bacterial Persistence pp 101–109Cite as

Single-Cell Detection and Collection of Persister Bacteria in a Directly Accessible Femtoliter Droplet Array

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1333))

Abstract

A directly accessible femtoliter droplet array as a platform for single-cell detection and collection of persister bacteria is described. Device microfabrication, femtoliter droplet array formation and concomitant enclosure of single cells, long-term culture and observation of single cells in droplets, and collection of identified persisters from single droplets are described in detail.

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Author information

Authors and Affiliations

  1. Okazaki Institute for Integrative Bioscience, Institute for Molecular Science, National Institutes of Natural Sciences, Aichi, 444-8787, Japan

    Ryota Iino

  2. Department of Functional Molecular Science, School of Physical Sciences, The Graduate University for Advanced Studies (SOKENDAI), Kanagawa, 240-0193, Japan

    Ryota Iino

  3. Technical Division, Institute of Scientific and Industrial Research, Osaka University, Osaka, Japan

    Shouichi Sakakihara

  4. Laboratory of Microbiology and Infectious Diseases, Institute of Scientific and Industrial Research, Osaka University, Osaka, Japan

    Yoshimi Matsumoto & Kunihiko Nishino

Authors
  1. Ryota Iino
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  2. Shouichi Sakakihara
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  3. Yoshimi Matsumoto
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  4. Kunihiko Nishino
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Corresponding author

Correspondence to Ryota Iino .

Editor information

Editors and Affiliations

  1. Department of Microbial and Molecular Systems, KU Leuven - University of Leuven, Heverlee, Belgium

    Jan Michiels

  2. Department of Microbial and Molecular Systems, KU Leuven - University of Leuven, Heverlee, Belgium

    Maarten Fauvart

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Cite this protocol

Iino, R., Sakakihara, S., Matsumoto, Y., Nishino, K. (2016). Single-Cell Detection and Collection of Persister Bacteria in a Directly Accessible Femtoliter Droplet Array. In: Michiels, J., Fauvart, M. (eds) Bacterial Persistence. Methods in Molecular Biology, vol 1333. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2854-5_9

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  • DOI: https://doi.org/10.1007/978-1-4939-2854-5_9

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2853-8

  • Online ISBN: 978-1-4939-2854-5

  • eBook Packages: Springer Protocols

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