Abstract
The present method quantifies the number of slow-growing bacteria leading to antibiotic persistence in a clonal population. First, it enables discriminating between slow growers that are generated by exposure to a stress signal (Type I persisters) and slow growers that are continuously generated during exponential growth (Type II persisters). Second, the method enables determining the amount of slow growers in a culture.
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Acknowledgments
The work was supported by the European Research Council (Starting Grant no. 260871) and the Israel Science Foundation (no. 592/10). I.L.R. acknowledges support from the Maydan Foundation.
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Levin-Reisman, I., Balaban, N.Q. (2016). Quantitative Measurements of Type I and Type II Persisters Using ScanLag. In: Michiels, J., Fauvart, M. (eds) Bacterial Persistence. Methods in Molecular Biology, vol 1333. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2854-5_7
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DOI: https://doi.org/10.1007/978-1-4939-2854-5_7
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2853-8
Online ISBN: 978-1-4939-2854-5
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