Skip to main content
SpringerLink
Log in
Book cover

Bacterial Persistence pp 177–187Cite as

Analysis of Macrophage-Induced Salmonella Persisters

  • Protocol

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1333))

Abstract

A small subpopulation of non-replicating, multidrug-tolerant bacteria is present within clonal populations of many bacterial species. Known as persisters, these bacteria are probably the cause of relapsing infections such as typhoid fever. Formation of non-growing Salmonella persisters is stimulated by macrophage phagocytosis. This chapter outlines methods to identify and study persisters resulting from interactions between bacterial pathogens and their hosts. We use their antibiotic tolerance for isolation and enumeration and developed a method to study the heterogeneity of growth within clonal populations through single-cell analysis.

This is a preview of subscription content, log in via an institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Helaine S, Cheverton AM, Watson KG, Faure LM, Matthews SA, Holden DW (2014) Internalization of Salmonella by macrophages induces formation of nonreplicating persisters. Science 343(6167):204–208. doi:10.1126/science.1244705

    Article  CAS  PubMed  Google Scholar 

  2. Helaine S, Thompson JA, Watson KG, Liu M, Boyle C, Holden DW (2010) Dynamics of intracellular bacterial replication at the single cell level. Proc Natl Acad Sci U S A 107(8):3746–3751. doi:10.1073/pnas.1000041107

    Article  PubMed Central  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Section of Microbiology, Medical Research Council Centre for Molecular Bacteriology and Infection, Imperial College London, South Kensington Campus, Armstrong Road, London, SW7 2AZ, UK

    Robert A. Fisher, Angela M. Cheverton & Sophie Helaine

Authors
  1. Robert A. Fisher
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Angela M. Cheverton
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sophie Helaine
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sophie Helaine .

Editor information

Editors and Affiliations

  1. Department of Microbial and Molecular Systems, KU Leuven - University of Leuven, Heverlee, Belgium

    Jan Michiels

  2. Department of Microbial and Molecular Systems, KU Leuven - University of Leuven, Heverlee, Belgium

    Maarten Fauvart

Rights and permissions

Reprints and permissions

Copyright information

© 2016 Springer Science+Business Media New York

About this protocol

Cite this protocol

Fisher, R.A., Cheverton, A.M., Helaine, S. (2016). Analysis of Macrophage-Induced Salmonella Persisters. In: Michiels, J., Fauvart, M. (eds) Bacterial Persistence. Methods in Molecular Biology, vol 1333. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2854-5_15

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-2854-5_15

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2853-8

  • Online ISBN: 978-1-4939-2854-5

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation