Visualization of Actin Cytoskeletal Dynamics in Fixed and Live Drosophila Egg Chambers
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Visualization of actin cytoskeletal dynamics is critical for understanding the spatial and temporal regulation of actin remodeling. Drosophila oogenesis provides an excellent model system for visualizing the actin cytoskeleton. Here, we present methods for imaging the actin cytoskeleton in Drosophila egg chambers in both fixed samples by phalloidin staining and in live egg chambers using transgenic actin labeling tools.
KeywordsActin cytoskeleton Oogenesis Nurse cells Microscopy Drosophila
We would like to thank Andrew Spracklen for his work on the live imaging protocols as well as the actin fixation protocol, and Ding Ye and Fang Lin for advice on using low-melt agarose to embed live egg chambers for imaging. This work was funded by National Science Foundation MCB-1158527.
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